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When this question was first posed in the s it was becoming a relatively simple exercise to isolate DNA and then randomly wlmen it up using chemical or mechanical means. Unfortunately, this random cutting was not a satisfactory way to obtain smaller pieces of DNA, since it womne impossible to tell what the original order of the DNA fragments were, an important point maxeira the specific order of Madiera is essential for its function. The biologists maceira stuck. A breakthrough was needed. As with many of Nude women in madeira important discoveries in biology, it was the study of bacteria that yielded this breakthrough.
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These restriction endonucleases provided biologists with a tool to study and manipulate DNA by enabling the generation of consistently sized DNA fragments. They are now used for a wide range of applications, including cloning, Southern hybridization analysis, DNA sequencing and global gene expression analysis SAGE. Their importance to biotechnology is apparent when looking through the catalogue of any company that supplies reagents for molecular biological work-there are pages and pages devoted to listing different restriction enzymes.
Lists that continue to grow as more enzymes are discovered. Truth be told, many recombinant DNA technologies, which the field of biotechnology heavily relies on, are unlikely to have been developed without the discovery of restriction enzymes. Restriction endonucleases were discovered during experiments to determine the ability of a bacteriophage the name given to viruses that infect bacteria to infect two different laboratory strains of Escherichia coli called strain B and strain K . In these experiments, bacteriophages were incubated with the different strains of E.
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